Biological reconstruction is still the preferred method to reconstruct bone defects caused by bone tumor resection in young patients. The removal of the tumor including a required margin is 3D virtually planned in which the information of different imaging modalities, such as CT, MRI and PET, is combined. The surgical resection plan is translated to the operation room by designing patient specific surgical guides that uniquely fit the patient anatomy and indicate the virtually planned resection. To validate the use of these guides surgical navigation is used during surgery. Data from a digital bonebank, that contains 3D models of all available allograft bones, is used to select the best matching allograft bone and to design resection guides for accurate allograft preparation during surgery.